PH1783 | DEPC水 无酶无菌水 DEPC Water (DNase/RNase free)

产品简介

DEPC水是用DEPC(diethypyrocarbonate，焦碳酸二乙酯)处理过并经高温高压消毒的Milli-Q纯水。经检测不含RNase、DNase和proteinase。

DEPC水可以用于RNA沉淀的溶解，含有RNA的各种反应体系如反转录、siRNA的退火等，以及其它各种要求无RNase、DNase和proteinase的反应体系。

DEPC水也叫无酶无菌水，本制品经高温高压灭菌处理，基本上不含DEPC，99.99%以上是水。

保存条件

常温运输与保存，有效期12个月

使用方法

根据实验需求将DEPC水添加到反应体系中。

注意事项

1.如果每次的使用量很小，可以适当分装后再使用。1.含tris的溶液不能使用DEPC进行处理

2.高压灭菌会使DEPC失活，但会残留轻微的分解后的气味，因此有气味不代表DEPC水失效过期

3.如需分装，建议在超净工作台内分装成小份后保存，避免污染

4.为了操作人员的安全和健康，请穿实验服并戴一次性手套操作

Diethyl pyrocarbonate (DEPC), also called diethyl dicarbonate (IUPAC name), is used in the laboratory to inactivate RNase enzymes in water and on laboratory utensils. It does so by the covalent modification of histidine (most strongly), lysine, cysteine, and tyrosine residues.

DEPC-treated (and therefore RNase-free) water is used in handling of RNA in the laboratory to reduce the risk of RNA being degraded by RNases.

Water is usually treated with 0.1% v/v DEPC for at least 2 hours at 37 °C and then autoclaved (at least 15 min) to inactivate traces of DEPC. Inactivation of DEPC in this manner yields CO2 and ethanol. Higher concentrations of DEPC are capable of deactivating larger amounts of RNase, but remaining traces or byproducts may inhibit further biochemical reactions such as in vitro translation. Furthermore, chemical modification of RNA such as carboxymethylation is possible when traces of DEPC or its byproducts are present, resulting in impaired recovery of intact RNA even after buffer exchange (after precipitation).